导师姓名：马翠萍

出生年月：1975.07

职       称：教授

学历（学位）：博士研究生

导师类别：博士生导师、硕士生导师

行政职务：betway西汉姆联官方官网教务处处长

招生专业：生物与医药、海洋化学、轻工技术与工程、分析生物技术、生物工程

联系方式：mcp169@163.com

一、个人简介

马翠萍，二级教授，现任betway西汉姆联官方官网教务处处长，博士生导师，无党派，留学归国人员，第十四届山东省人大代表，青岛市政协常委，全国普通高等公司本科公司产品评估专家，中国高等教育学会教育评估分会第七届理事会理事，山东省高层次人才、省级教学名师、省级优秀研究生指导教师，青岛青年先锋团队学术带头人。兼任中国-阿联酋国际合作联合实验室、山东省生物化学工程重点实验室、青岛核酸快检工程研究中心和青岛市核酸快检重点实验室主任，山东省科学技术协会代表。

主持国家自然科学基金、山东省重大基础研究项目等20余项；以第一或通讯作者在JACS等国际期刊发表SCI论文130余篇；以首位完成人获山东省自然科学奖二等奖1项、山东省高等公司优秀科研成果奖3项、青岛市科技进步奖二等奖1项。申请发明专利19件，已授权10件，PCT专利2件。积极推进科研成果转化，成功研发30余款基因检测产品，并投放市场。国内首批、省内首家推出新冠病毒基因检测试剂盒；研发食品核酸检测试剂盒被2022北京冬奥会选用，省内唯一。主持或参与省级及以上教研教改项目10余项，以首位获省教学成果特等奖1项，中国石油和化工公司产品优秀成果一等奖1项，山东省教师教学创新大赛三等奖1项，主讲省一流课程《生命安全与分析》。

二、研究方向

1. 基因检测新技术研究

2. 纳米生物分析和生物传感新方法研究

3. 生化检测新方法在食品安全和分子诊断等领域的应用

4. 合成生物学

三、科研项目及奖励

1. 国家自然科学基金面上项目，No. 31670868，等温扩增“一步法”RNA检测新技术研究，主持。

2. 国家自然基金青年科学基金项目，No. 21307064，乙酰胆碱酯酶核酸适体快速筛选的新方法研究，主持。

3. 国家自然科学基金面上项目，No. 31170758，一种基于蛋白质二级结构模块重组建立基因文库的新方法，主持。

4. 山东省自然科学基金重点项目，No. ZR2020KH030，新型冠状病毒核酸的快速提取及一体化快速检测方法研究，主持。

5. 山东省自然科学基金重大基础研究项目，No. ZR2017ZC0123，基于微流控芯片和核酸等温扩增的微纳生物分析器件的研制，主持。

6. 山东省自然科学奖二等奖，第1位，2023.12

7. 山东省高等公司科学技术奖二等奖，第1位，2018.10

8. 山东高等公司优秀科研成果奖二等奖，第1位，2011.12

9. 青岛市科技进步奖二等奖，第1位，2025.11

四、教学项目及奖励

1. 主持山东省普通高等公司实验教学和教学实验室建设研究项目，“一平台二层级四协同”的虚拟仿真实验教学管理模式创新与实践，2024.04

2. 主讲《生命安全与分析》获山东省一流本科课程，线上线下混合式课程，2024.03

3. 主持山东省高等教育学会高等教育研究专项重点课题，高等教育数字化背景下地方高校基层教学组织新形态研究探索与实践，2023.03-2025.03

4. 主持山东省本科教学改革研究重点项目，No. Z2022067，高等教育数字化背景下地方高校基层教学组织新形态建设研究与实践，2022.12-2024.11

5. 主持betway西汉姆联官方官网研究生教育课程思政示范课程，2022KCSZ15，生物科学前沿讲座，2022年

6. 主编《生物医学分析化学》，科学出版社，2023年

7. 山东省第十届高等教育省级教学成果奖特等奖，第1位，2025.12

8. 山东省2025年度普通高等公司教师教学创新大赛三等奖，2025.06

9. 中国石油和化工公司产品优秀成果奖一等奖，第1位，2024.10

10. 2021年度实验技术成果奖一等奖，第1位，2021.12

11. betway西汉姆联官方官网首届教书育人楷模，2021.09

12. 第五届betway西汉姆联官方官网年度人物，2020.09

13. betway西汉姆联官方官网教育先锋，2018.04

14. betway西汉姆联官方官网十大杰出青年，2016.05

五、代表性论文

1. Chao Shi, Xiaotong Shen, Shuyan Niu, Cuiping Ma. Innate reverse transcriptase activity of DNA polymerase for isothermal RNA direct detection. Journal of the American Chemical Society, 2015, 137, 13804-13806

2. Jiaqi Liu, Xiangyu Chen, Xue Feng, Jiayu Zheng, Chao Shi, Yong Li, Cuiping Ma. Beyond electrostatic adsorption: Zr-functionalized magnetic nanoparticles achieve selective and enhanced small RNA extraction from Bacteria and plasma, Separation and Purification Technology, 2026, 389, 136945

3. Xiaoguang Lu, Qianyue Jia, Yaning Zheng, Zishan Qin, Yong Li, Chao Shi, Cuiping Ma. A novel method of droplet generation based on the self-propelling of water phase and its potential application in multiplexing continuous flow PCR, Biosensors and Bioelectronics, 2025, 279, 117400

4. Yong Li, Yanwen Qi, Jiaqi Liu, Pengyu Wang, Jiayu Zheng, Xiangyu Chen, Ye Wang, Xiaowen Zhao, Yingqiu Xie, Chao Shi, and Cuiping Ma. Antibiotic-Modified Nanoparticles Combined with Lysozyme for Rapid Extraction of Pathogenic Bacteria DNA in Blood, Analytical Chemistry, 2025, 97(11), 6201-6210

5. Yaofang Fan, Yuxin Xue, Haoran Wang, Yu Liao, Zhiying Gao, Xiujuan Wang, Chao Shi, Cuiping Ma. Effect of 3D branched crystalline carbon nitride on nucleic acid amplification techniques in food samples, Food Control, 2025, 175, 111299

6. Jinling Cui, Anning Tian, Haoran Wang, Yanan Yu, Jingwen Hao, Lei Wang,  Chao Shi, Cuiping Ma. Hydrogel loop-mediated isothermal amplification for ultra-fast diagnosis of Helicobacter pylori in stool samples without nucleic acid extraction, Analytica Chimica Acta, 2025, 1333, 343384

7. Yilin Wang, Fengyi Li, Xiudan Wang, Cuiping Ma. Integrating hypoxanthine and K value for reliable and rapid freshness assessment in marine fish, Food Chemistry, 2025, 470, 142630

8. Yaofang Fan, Yu Liao, Zhiying Gao, Haoran Wang, Yong Li, Chao Shi, Cuiping Ma. N-Doped Porous Carbon Synergistic Freezing-Induced DNA with Catalyzed Hairpin Assembly Enables Electrochemical One-Pot Detection of Pathogen in Food Samples. Journal of Agricultural and Food Chemistry, 2025, 73(7), 4342-4352

9. Xiujuan Wang, Binghui Shi, Cengceng Xia, Mengnan Hou, Jingying Wang, Anning Tian, Chao Shi, Cuiping Ma. Poly-L-lysine functionalized silica membrane-enhanced colorimetric loop-mediated isothermal amplification for sensitive and rapid detection of Vibrio parahaemolyticus, Talanta, 2025, 288, 127744

10. Yang Li, Jiachen Sun, Qing Wang, Chang Su, Xiguang Chen, Cuiping Ma. Xuecheng Yang, Chao Feng, Chao Shi, Lysis-Free Isolation and Direct Amplification of Pathogenic Bacterial DNA Using Diatom Frustules, Analytical Chemistry, 2024, 96(22), 9113-9121

11. Yilin Wang, Xiaoyun Zhang, Maole Wei, Huigang Kang, Zhenqiang Zhang, Xiudan Wang, Cuiping Ma. Green manufacturing of a hypoxanthine enzyme sensor for fish freshness based on modified nitrocellulose surface with chito-oligosaccharide, Talanta, 2024, 274, 126007

12. Yong Li, Xin Zhang, Yu Liao, Chao Shi, Ye Wang, Xiaofeng Mu, Yingqiu Xie, Cuiping Ma. Engineering of a Chimeric Template Triggers RNase H-Based Isothermal Amplification Approach for Sensitive Detection of Pathogen RNA, Analytical Chemistry, 2023, 95(49), 18249-18257

13. Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma. Boron Nitride Nanoplate-Based Improvement of the Specificity and Sensitivity in Loop-Mediated Isothermal Amplification for Vibrio parahaemolyticus Detection. Analytica Chimica Acta, 2023, 1280, 341851

14. Yong Li, Xiangning Han, Xiaofeng Mu, Ye Wang, Chao Shi, Cuiping Ma. Single-Walled Carbon Nanotubes-Based RNA Protection and Extraction Improves RT-PCR Sensitivity for SARS-CoV-2 Detection. Analytica Chimica Acta, 2023, 1238, 340639

15. Yong Li, Chunyu Yan, Jiao Chen, Yunyun Lian, Yingqiu Xie, Amr Amin, Qianyi Wan, Chao Shi, Cuiping Ma. An all-in-one nucleic acid enrichment and isothermal amplification platform for rapid detection of Listeria monocytogenes. Food Control, 2022, 139, 109096

16. Xuejiao Wang, Yong Li, Mingyuan Zhao, Haixia Wang, Qianyi Wan, Chao Shi, Cuiping Ma. An Ultrafast Ratiometric Electrochemical Biosensor Based on Potential-Assisted Hybridization for Nucleic Acids Detection. Analytica Chimica Acta, 2022, 1211, 339915

17. Yong Li, Chunyu Yan, Jiao Chen, Yunyun Lian, Yingqiu Xie, Amr Amin, Qianyi Wan, Chao Shi, Cuiping Ma. An all-in-one nucleic acid enrichment and isothermal amplification platform for rapid detection of Listeria monocytogenes, Food Control 139 2022

18. Xiaokun Wang, Hezhen Shang, Cuiping Ma. Lingxin Chen, A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification, Analytical Chemistry, 2021, 93(24), 8493-8500

19. Chunyu Yan, Xiudan Wang, Xiaoli Zhao, Manman Wei, Chao Shi, Cuiping Ma. Development of a direct and visual isothermal method for meat adulteration detection in low resource settings. Food Chemistry, 2020, 319, 126542

20. Shuai Liu, Manman Wei, Xuejiao Wang, Chao Shi, Shaoping Kuang, Cuiping Ma. An ultrasensitive electrochemical DNA sensing strategy free from pre-immobilization via G-quadruplex based homogenous proximity hybridization. Talanta, 2020, 210, 120628

21. Shuai Liu, Manman Wei, Rui Liu, Shaoping Kuang, Chao Shi, Cuiping Ma. Lab in a Pasteur pipette: low-cost, rapid and visual detection of Bacillus cereu using denaturation bubble-mediated strand exchange amplification. Analytica Chimica Acta, 2019, 1080, 162-169

22. Rui Liu, Xiudan Wang, Xuejiao Wang, Yanjing Shi, Chao Shi, Wei Wang, Cuiping Ma. A simple isothermal nucleic acid amplification method for the effective on-site identification for adulteration of pork source in mutton. Food Control, 2019, 98, 297-302

23. Chao Shi, Fanjin Shang, Mei Pan, Sen Liu, Cuiping Ma. The isothermal amplification detection of double-stranded DNA based on a double-stranded fluorescence probe. Biosensors and Bioelectronics, 2016, 80, 54-58

24. Chao Shi, Qi Liu, Meiling Zhou, Haijie Zhao, Tao Yang, Cuiping Ma. Nicking endonuclease-mediated isothermal exponential amplification for double-stranded DNA detection. Sensors and Actuators B: Chemical, 2016, 222, 221-225

25. Cuiping Ma, Sen Liu, Chao Shi. Ultrasensitive detection of microRNAs based on hairpin fluorescence probe assisted isothermal amplification. Biosensors and Bioelectronics, 2014, 58, 57-60

26. Chao Shi, Qi Liu, Cuiping Ma, Wenwan Zhong. Exponential strand-displacement amplification for detection of microRNAs. Analytical Chemistry, 2014, 86, 336-339

27. Cuiping Ma, Chunhui Zhao, Yujie Ge, Chao Shi. Aptameric molecular switch for cascade signal amplification. Clinical Chemistry, 2012, 58(2), 384-390

28. Chao Shi, Yujie Ge, Hongxi Gu, Cuiping Ma. Highly sensitive chemiluminescent point mutation detection by circular strand-displacement amplification reaction. Biosensors and Bioelectronics, 2011, 26, 4697-4701

29. Cuiping Ma, Wenshuo Wang, Qing Yang, Chao Shi, Lijie Cao. Cocaine detection via rolling circle amplification of short DNA strand separated by magnetic beads. Biosensors and Bioelectronics, 2011, 26, 3309-3312

六、授权专利

1. 一种用于核酸扩增比色反应的缓冲液及其应用，ZL201811352744.4，

2. 一种改性硅藻土提取核酸的方法及应用，ZL202111407746.0

3. 一种非诊断目的的核酸扩增纸基可视化检测平台，ZL202010258681.7

4. 一种微流控芯片及可被密封的进样结构，ZL201921910484.8

5. 一种便携式核酸比色检测装置，ZL201920686855.2

6. 一种基于切刻酶的双链核酸等温扩增检测新方法，ZL201410529006.8

7. 一种人工改造功能蛋白的方法，ZL201510333715.3

8. 一种单引物引发的核酸恒温扩增方法，ZL201410324430.9

9. 一种基于指数发卡组装和比色快速检测核酸的方法，ZL201410034989.8

10. 一种多切刻酶位点介导的核酸恒温扩增检测方法，ZL201310573895.3